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goat anti human p63 tp73l polyclonal antibody (R&D Systems)

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R&D Systems goat anti human p63 tp73l polyclonal antibody

Evaluation of the antiviral activity of compound 0179841 in a human airway organoid model. ( A ) Establishment of HBEC-derived apical-out airway organoids. Representative white-light images of organoids cultured over the long term are presented, along with confocal images demonstrating the expression of respiratory epithelial markers. The confocal images illustrate the presence and spatial distribution of distinct cell types within the organoids, as identified by their specific markers: basal cells labeled with <t>P63</t> (purple), goblet cells labeled with MUC5AC (green), and ciliated cells labeled with acetylated tubulin (red). ( B–E ) Organoids were infected with RSV ON1-GFP at an MOI of 1 and cultured in media supplemented with 0.1% DMSO or compound 0179841 at the indicated concentrations (5, 1, and 0.5 µM) for 96 h. ( B ) The cell culture supernatant of infected organoids was collected, and a TCID 50 assay was performed. ( C ) The RSV ON1-GFP-infected organoids were visualized via microscopy. ( D ) The GFP intensity in five randomly selected fields per treatment was measured using ImageJ analysis software and normalized to the intensity in the DMSO condition. ( E ) The RSV ON1-GFP-infected organoids were visualized using a confocal microscope. The graphs shown are representative of three independent experiments. The differences between the experimental and DMSO groups were determined by Student’s t -test for comparing two groups of data (*** P < 0.001).

Goat Anti Human P63 Tp73l Polyclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+p63/Human+p63%2FTP73L+Antibody/pmc12817919-306-32-37
Average 93 stars, based on 32 article reviews

goat anti human p63 tp73l polyclonal antibody - by Bioz Stars, 2026-09

93/100 stars

Images

1) Product Images from "Molecular mechanism of resistance to lonafarnib conferred by mutations in the cysteine-rich region of respiratory syncytial virus fusion glycoprotein and discovery of a lonafarnib-derived antiviral PROTAC"

Article Title: Molecular mechanism of resistance to lonafarnib conferred by mutations in the cysteine-rich region of respiratory syncytial virus fusion glycoprotein and discovery of a lonafarnib-derived antiviral PROTAC

Journal: Journal of Virology

doi: 10.1128/jvi.01487-25

Figure Legend Snippet: Evaluation of the antiviral activity of compound 0179841 in a human airway organoid model. ( A ) Establishment of HBEC-derived apical-out airway organoids. Representative white-light images of organoids cultured over the long term are presented, along with confocal images demonstrating the expression of respiratory epithelial markers. The confocal images illustrate the presence and spatial distribution of distinct cell types within the organoids, as identified by their specific markers: basal cells labeled with P63 (purple), goblet cells labeled with MUC5AC (green), and ciliated cells labeled with acetylated tubulin (red). ( B–E ) Organoids were infected with RSV ON1-GFP at an MOI of 1 and cultured in media supplemented with 0.1% DMSO or compound 0179841 at the indicated concentrations (5, 1, and 0.5 µM) for 96 h. ( B ) The cell culture supernatant of infected organoids was collected, and a TCID 50 assay was performed. ( C ) The RSV ON1-GFP-infected organoids were visualized via microscopy. ( D ) The GFP intensity in five randomly selected fields per treatment was measured using ImageJ analysis software and normalized to the intensity in the DMSO condition. ( E ) The RSV ON1-GFP-infected organoids were visualized using a confocal microscope. The graphs shown are representative of three independent experiments. The differences between the experimental and DMSO groups were determined by Student’s t -test for comparing two groups of data (*** P < 0.001).

Techniques Used: Activity Assay, Derivative Assay, Cell Culture, Expressing, Labeling, Infection, Microscopy, Software

Incubation:

Article Title: Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD.
Article Snippet: .. Tissue sections were then incubated with the following primary antibodies for one night at 4 °C in 3% BSA in PBS: rabbit anti-Arl13b (17711 1-ap, ProteinTech, 1:200); mouse anti-Muc5ac (NBP2-15196, Novus Biologicals, 1:100); rabbit antiMuc5B (E-AB-15988, Elabscience, 1:100); mouse anti-Acetylateda-tubulin (T6793, Sigma Aldrich, 1:1000); goat anti-P63 (AF1916, R&D systems, 1:100); rabbit anti-Gli1 (HPA065172, Sigma Aldrich, 1 mg/mL); rabbit anti-Gli2 (HPA074275, Sigma Aldrich, 0,4 mg/mL); rabbit anti-Gli3 (HPA005534, Sigma, 0,6 mg/mL); goat anti-Shh (AF464, R&D Systems); rabbit anti-Smoothened (E-AB-12925, Elabscience, 1:50) and rabbit anti-Patched1 (E-AB-10571, Elabscience, 1:100). ..

Article Title: Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD
Article Snippet: .. Tissue sections were then incubated with the following primary antibodies for one night at 4 °C in 3% BSA in PBS: rabbit anti-Arl13b (17711–1-ap, ProteinTech, 1:200); mouse anti-Muc5ac (NBP2-15196, Novus Biologicals, 1:100); rabbit anti-Muc5B (E-AB-15988, Elabscience, 1:100); mouse anti-Acetylated-α-tubulin (T6793, Sigma Aldrich, 1:1000); goat anti-P63 (AF1916, R&D systems, 1:100); rabbit anti-Gli1 (HPA065172, Sigma Aldrich, 1 μg/mL); rabbit anti-Gli2 (HPA074275, Sigma Aldrich, 0,4 μg/mL); rabbit anti-Gli3 (HPA005534, Sigma, 0,6 μg/mL); goat anti-Shh (AF464, R&D Systems); rabbit anti-Smoothened (E-AB-12925, Elabscience, 1:50) and rabbit anti-Patched1 (E-AB-10571, Elabscience, 1:100). ..

Article Title: Sonic hedgehog signalling as a potential endobronchial biomarker in COPD
Article Snippet: .. Samples were rehydrated with PBS and blocked with 10% PBS-BSA for 30 min at room temperature before incubation with the following primary antibodies overnight at 4 °C in 3% PBS-BSA: rabbit anti-Arl13b (17711–1-ap, ProteinTech, 1:200); mouse anti-Muc5ac (NBP2–15196, Novus Biologicals, 1:100); mouse anti-Acetylated-α-tubulin (T6793, Sigma Aldrich, 1:1000); goat anti-p63 (AF1916, R&D systems, 1:100); rabbit anti-pancytokeratin (E-AB-33599; Clinisciences, 1:100); mouse anti-vimentin (M0725; Dako, 1:100); rabbit anti-Gli1 (HPA065172, Sigma Aldrich, 1 μg/mL); rabbit anti-Gli2 (HPA074275, Sigma Aldrich, 0,4 μg/mL); goat anti-Shh (AF464, R&D systems, 1:100); rabbit anti-Ptch1 (E-AB-10571, Clinisciences, 1:100); mouse anti-Hhip (WH0064399M1, Sigma-Aldrich, 1:100). ..

Image Search Results

Journal: Journal of Virology

doi: 10.1128/jvi.01487-25

Figure Lengend Snippet: Evaluation of the antiviral activity of compound 0179841 in a human airway organoid model. ( A ) Establishment of HBEC-derived apical-out airway organoids. Representative white-light images of organoids cultured over the long term are presented, along with confocal images demonstrating the expression of respiratory epithelial markers. The confocal images illustrate the presence and spatial distribution of distinct cell types within the organoids, as identified by their specific markers: basal cells labeled with P63 (purple), goblet cells labeled with MUC5AC (green), and ciliated cells labeled with acetylated tubulin (red). ( B–E ) Organoids were infected with RSV ON1-GFP at an MOI of 1 and cultured in media supplemented with 0.1% DMSO or compound 0179841 at the indicated concentrations (5, 1, and 0.5 µM) for 96 h. ( B ) The cell culture supernatant of infected organoids was collected, and a TCID 50 assay was performed. ( C ) The RSV ON1-GFP-infected organoids were visualized via microscopy. ( D ) The GFP intensity in five randomly selected fields per treatment was measured using ImageJ analysis software and normalized to the intensity in the DMSO condition. ( E ) The RSV ON1-GFP-infected organoids were visualized using a confocal microscope. The graphs shown are representative of three independent experiments. The differences between the experimental and DMSO groups were determined by Student’s t -test for comparing two groups of data (*** P < 0.001).

Article Snippet: The organoids were fixed in parallel in 4% PFA, permeabilized with 0.5% Triton X-100 (1 h), blocked with 5% BSA (Macklin, cat#B824162; 2 h), and incubated overnight at 4°C with primary antibodies: Goat anti-Human p63/TP73L polyclonal antibody (R&D Systems, cat#AF1916), Rabbit anti-Human Mucin 5AC polyclonal antibody (Abways, cat#CY6826), and Mouse anti-acetylated tubulin monoclonal antibody (Sigma-Aldrich, cat#T7451).

Techniques: Activity Assay, Derivative Assay, Cell Culture, Expressing, Labeling, Infection, Microscopy, Software

a , Representative IHC staining on human SCLC biopsies for given markers with sample name indicated. One row = one tumour. Scale bar, 25 μm. b , Venn diagram depicting number of human SCLC biopsies (n = 119 total) staining positive for ASCL1, NEUROD1, POU2F3, or lacking all markers (“Subtype-Neg”). c , Representative co-immunofluorescence (co-IF) staining on human SCLC biopsies (n = 28 total) for DAPI (nuclei, blue), ASCL1 (yellow), NEUROD1 (purple) or POU2F3 (green). Individual channels (top) and an overlay without DAPI (bottom) are shown. Scale bars, 50 μm. Yellow arrows indicate colocalization of markers. d , Representative co-IF staining on patient-derived-xenografts (n = 2 distinct models) for DAPI (nuclei, blue), ASCL1 (green), NEUROD1 (purple) or POU2F3 (red). Individual channels (left) and an overlay without DAPI (right) are shown. Yellow arrows and insets (a-b) emphasize co-expressing cells (bottom). Scale bars, 75 μm. e , Co-IF staining of tracheal and lung epithelium pre-(Day 0) and post-naphthalene (Day 3, 5, 10) for indicated markers. DAPI marks nuclei (blue), P63 and KRT5 (basal), CCSP (club), and KRT13 (hillock). K5-Cre was not administered to these mice, but the timepoint of typical K5-Cre administration in autochthonous GEMMs is indicated for ease of interpretation of cell types present at the biologically-relevant timepoint for tumour experiments. Scale bars, 10 µm. f , Quantification of positive cells per mm of epithelium in the trachea or lung epithelium, with dashed line at Day 3 (timepoint K5-Cre is typically administered in autochthonous GEMMs), showing the frequency of cells present at the theoretical time of K5-Cre administration. Statistics performed on frequency values from n = 12–58 regions of tracheal epithelium or n = 13–50 regions of lung epithelium per timepoint. Error represents mean +/- SEM. Quantification of n = ~4–12 total mm lung or n = ~13–51 total mm tracheal epithelium per timepoint per stain from n = 1–2 mice at Day 0, n = 2 mice at Day 1.5, n = 2 mice at Day 2, n = 4 mice at Day 3, n = 2 mice at Day 5, and n = 2 mice at Day 10.

Journal: Nature

Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer

doi: 10.1038/s41586-025-09503-z

Figure Lengend Snippet: a , Representative IHC staining on human SCLC biopsies for given markers with sample name indicated. One row = one tumour. Scale bar, 25 μm. b , Venn diagram depicting number of human SCLC biopsies (n = 119 total) staining positive for ASCL1, NEUROD1, POU2F3, or lacking all markers (“Subtype-Neg”). c , Representative co-immunofluorescence (co-IF) staining on human SCLC biopsies (n = 28 total) for DAPI (nuclei, blue), ASCL1 (yellow), NEUROD1 (purple) or POU2F3 (green). Individual channels (top) and an overlay without DAPI (bottom) are shown. Scale bars, 50 μm. Yellow arrows indicate colocalization of markers. d , Representative co-IF staining on patient-derived-xenografts (n = 2 distinct models) for DAPI (nuclei, blue), ASCL1 (green), NEUROD1 (purple) or POU2F3 (red). Individual channels (left) and an overlay without DAPI (right) are shown. Yellow arrows and insets (a-b) emphasize co-expressing cells (bottom). Scale bars, 75 μm. e , Co-IF staining of tracheal and lung epithelium pre-(Day 0) and post-naphthalene (Day 3, 5, 10) for indicated markers. DAPI marks nuclei (blue), P63 and KRT5 (basal), CCSP (club), and KRT13 (hillock). K5-Cre was not administered to these mice, but the timepoint of typical K5-Cre administration in autochthonous GEMMs is indicated for ease of interpretation of cell types present at the biologically-relevant timepoint for tumour experiments. Scale bars, 10 µm. f , Quantification of positive cells per mm of epithelium in the trachea or lung epithelium, with dashed line at Day 3 (timepoint K5-Cre is typically administered in autochthonous GEMMs), showing the frequency of cells present at the theoretical time of K5-Cre administration. Statistics performed on frequency values from n = 12–58 regions of tracheal epithelium or n = 13–50 regions of lung epithelium per timepoint. Error represents mean +/- SEM. Quantification of n = ~4–12 total mm lung or n = ~13–51 total mm tracheal epithelium per timepoint per stain from n = 1–2 mice at Day 0, n = 2 mice at Day 1.5, n = 2 mice at Day 2, n = 4 mice at Day 3, n = 2 mice at Day 5, and n = 2 mice at Day 10.

Article Snippet: The primary antibodies included anti-mouse ASCL1 (BD Pharmingen; 556604) 1:25, anti-rabbit ASCL1 (Abcam; ab211327) 1:100, anti-goat NEUROD1 (R&D Systems; AF2746) 1:50, anti-rabbit NEUROD1 (Abcam; ab213725) 1:200, POU2F3 (Sigma; HPA019652) 1:100, anti-rabbit CCSP/SCGB1A1 (MilliporeSigma; 07-623) 1:75, anti-rat KRT8 (Developmental Studies Hybridoma Bank; TROMA-I) 1:100, anti-mouse FOXJ1 (eBioscience; 14-9965-80) 1:100, anti-mouse KI67 (BD Pharmingen; BDB556003) 1:100, anti-goat P63 (R&D Systems; AF1916) 1:40, anti-chicken mCherry/tdTomato (Sigma; AB356481 ) 1:100, anti-rabbit KRT13 (Abcam; ab92551) 1:200, anti-guinea-pig KRT13 (Origene; BP5076) 1:100, anti-rabbit KRT5 (BioLegend; 905501) 1:200, anti-mouse KRT5 (GeneTex; GTX60580) 1:100 and anti-rabbit CGRP (Sigma; C8198) 1:100.

Techniques: Immunohistochemistry, Staining, Immunofluorescence, Derivative Assay, Expressing

a , Representative flow cytometry data from tracheal basal cells analyzed with a live/dead marker, ITGA6, indicated basal markers (KRT5, NGFR), and/or hillock marker (KRT13). Gating strategy includes progressive gating on cell size, singlets, and live cells (top row), followed by analysis of ITGA6 on Y-axis and indicated control (no primary) or basal marker on X-axis. Percentage of total live cells indicated in each quadrant. Quantification in upper right indicates the percentage of ITGA6 + cells specifically that co-express another basal or hillock marker based on mean fluorescence of that marker relative to all live cells. b , Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font = condition selected for subsequent allografting. For gel source data, see Supplementary Fig. . c , Co-immunofluorescence staining (Co-IF) of wildtype “WT” basal organoids (collected within ~2 weeks of CMV-Cre transformation) and CMV-Cre-transformed basal-derived organoids (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE, green). Positive controls (bottom panel) are an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid per genotype (bottom). Number of organoids quantified is labeled. One-way ANOVA with post-hoc Tukey’s correction. Exact p- values in figure. Error is mean ± SD. d , Co-IF of WT basal organoids and CMV-Cre-transformed basal-derived organoids (RPM, RPR2, RPMA): Left panel) DAPI (nuclei, blue), NEUROD1 (neuronal, green), P63 (basal, yellow) and KRT8 (luminal basal, red). Positive control (+) for NEUROD1 is a murine olfactory neuroblastoma tumour and for basal markers is a murine squamous lung tumour. Right panel) DAPI (nuclei, blue), FOXJ1 (ciliated, purple), CCSP (SCGB1A1, club, green) and KRT8 (luminal basal, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is a murine squamous lung tumour. e , Co-IF of WT basal organoids and CMV-Cre-transformed basal-derived organoids (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft, green). Positive control (+) is a murine olfactory neuroblastoma tumour. f , Co-IF staining of WT basal organoids and RPM basal-derived organoids for DAPI (nuclei) or indicated basal markers. Positive control is murine squamous lung tumour (+). All co-IF scale bars, 150 μm. Staining results in (c-f) are representative of organoids collected from three or more independent experiments per genotype, all with similar results.

Journal: Nature

Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer

doi: 10.1038/s41586-025-09503-z

Figure Lengend Snippet: a , Representative flow cytometry data from tracheal basal cells analyzed with a live/dead marker, ITGA6, indicated basal markers (KRT5, NGFR), and/or hillock marker (KRT13). Gating strategy includes progressive gating on cell size, singlets, and live cells (top row), followed by analysis of ITGA6 on Y-axis and indicated control (no primary) or basal marker on X-axis. Percentage of total live cells indicated in each quadrant. Quantification in upper right indicates the percentage of ITGA6 + cells specifically that co-express another basal or hillock marker based on mean fluorescence of that marker relative to all live cells. b , Recombination PCR for RPM basal organoids for indicated alleles pre- and post-treatment with TAT-Cre or Ad-CMV-Cre at two concentrations (2.5e7 or 5e7 pfu). *Organoids subject to spinoculation with CMV-Cre virus. Red font = condition selected for subsequent allografting. For gel source data, see Supplementary Fig. . c , Co-immunofluorescence staining (Co-IF) of wildtype “WT” basal organoids (collected within ~2 weeks of CMV-Cre transformation) and CMV-Cre-transformed basal-derived organoids (RPM, RPR2, RPMA) for DAPI (nuclei, blue), KI67 (proliferation, orange), and ASCL1 (NE, green). Positive controls (bottom panel) are an RPR2 SCLC lung tumour. Quantification via CellProfiler of KI67 positivity per organoid per genotype (bottom). Number of organoids quantified is labeled. One-way ANOVA with post-hoc Tukey’s correction. Exact p- values in figure. Error is mean ± SD. d , Co-IF of WT basal organoids and CMV-Cre-transformed basal-derived organoids (RPM, RPR2, RPMA): Left panel) DAPI (nuclei, blue), NEUROD1 (neuronal, green), P63 (basal, yellow) and KRT8 (luminal basal, red). Positive control (+) for NEUROD1 is a murine olfactory neuroblastoma tumour and for basal markers is a murine squamous lung tumour. Right panel) DAPI (nuclei, blue), FOXJ1 (ciliated, purple), CCSP (SCGB1A1, club, green) and KRT8 (luminal basal, red). Positive control (+) control for FOXJ1 and CCSP is airway from a normal mouse lung, and for KRT8 is a murine squamous lung tumour. e , Co-IF of WT basal organoids and CMV-Cre-transformed basal-derived organoids (RPM, RPR2, RPMA) for DAPI (nuclei, blue) and POU2F3 (tuft, green). Positive control (+) is a murine olfactory neuroblastoma tumour. f , Co-IF staining of WT basal organoids and RPM basal-derived organoids for DAPI (nuclei) or indicated basal markers. Positive control is murine squamous lung tumour (+). All co-IF scale bars, 150 μm. Staining results in (c-f) are representative of organoids collected from three or more independent experiments per genotype, all with similar results.

Techniques: Flow Cytometry, Marker, Control, Fluorescence, Virus, Immunofluorescence, Staining, Transformation Assay, Derivative Assay, Labeling, Positive Control

a , T7 endonuclease assay on transformed RPM and RPMA basal organoids after lentiviral infection of LCV2 with sg Ctrl or sgPten . Expected products of digestion with editing are 671 and 239 bp. b , Immunoblot of pAKT (Ser473) and total AKT with HSP90 as loading control on transformed RPM and RPMA basal organoids with LCV2-sg Ctrl or - sgPten . c , Quantification of tumour volume (mm^3) over time (weeks) in RPM (top) and RPMA (bottom) sgCtrl (passage 1=orange, solid; passage 2=green, dashed), and sgPten (passage 1=blue, solid; passage 2=purple, dashed, passage 3=purple, dotted), basal organoid allografts. Exact number of tumours quantified indicated in figure. d , IHC of RPM and RPMA basal-organoid-derived tumours infected with LCV2- sgControl ( sgCtrl ) or sgPten for indicated markers (left). H-score quantification for indicated proteins (right) in RPM and RPMA “Ctrl” (parental and sgCtrl -infected tumours, orange) and “ sgPten ” tumours (purple) with SCLC-dominant histopathology (> 50% of tumour region analyzed is SCLC). Quantification of NSCLC-dominant tumours (only in RPMA) included on far right (both “Control” and “ sgPten ” tumours). Exact number of tumours quantified from n = 5–6 mice per genotype indicated in figure. Multi-passage tumours included. e , Representative H&E and IHC for indicated markers in RPM and RPMA sg Ctrl and sgPten tumours with SCLC histopathology versus regions of NSCLC histology including adenocarcinoma (Adeno), adeno-squamous carcinoma (Adeno-squamous), or Squamous differentiation. H-score quantification for indicated proteins (right) in RPM and RPMA “Ctrl” (parental and sgControl -infected tumours, orange) and “ sgPten ” tumours (purple) with SCLC-dominant histopathology (> 50% of tumour region analyzed). Quantification of NSCLC-dominant tumours (only found in RPMA) included on the far right (both “Control” and “ sgPten ” tumours). Exact number of tumours quantified from n = 2–6 mice per genotype indicated in figure. Multi-passage tumours included. f , Stacked bar chart has average proportions of indicated histopathologies in individual RPM and RPMA control or sgPten -tumours. Exact number of tumours analyzed indicated above bars. Error bars, mean ± SEM. Histopathologies determined via analysis of H&E and NKX2-1, P63, KRT5, and SCLC subtype marker staining. LCNEC is large-cell neuroendocrine carcinoma. For gel source data for (a,b), see Supplementary Fig. . All scale bars, 50 μm. All statistical tests are one-way ANOVA tests with Fisher’s LSD multiple comparisons; **** p < 0.0001, ns=not significant= p > 0.05, and other exact p- values indicated in figure.

Journal: Nature

Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer

doi: 10.1038/s41586-025-09503-z

Figure Lengend Snippet: a , T7 endonuclease assay on transformed RPM and RPMA basal organoids after lentiviral infection of LCV2 with sg Ctrl or sgPten . Expected products of digestion with editing are 671 and 239 bp. b , Immunoblot of pAKT (Ser473) and total AKT with HSP90 as loading control on transformed RPM and RPMA basal organoids with LCV2-sg Ctrl or - sgPten . c , Quantification of tumour volume (mm^3) over time (weeks) in RPM (top) and RPMA (bottom) sgCtrl (passage 1=orange, solid; passage 2=green, dashed), and sgPten (passage 1=blue, solid; passage 2=purple, dashed, passage 3=purple, dotted), basal organoid allografts. Exact number of tumours quantified indicated in figure. d , IHC of RPM and RPMA basal-organoid-derived tumours infected with LCV2- sgControl ( sgCtrl ) or sgPten for indicated markers (left). H-score quantification for indicated proteins (right) in RPM and RPMA “Ctrl” (parental and sgCtrl -infected tumours, orange) and “ sgPten ” tumours (purple) with SCLC-dominant histopathology (> 50% of tumour region analyzed is SCLC). Quantification of NSCLC-dominant tumours (only in RPMA) included on far right (both “Control” and “ sgPten ” tumours). Exact number of tumours quantified from n = 5–6 mice per genotype indicated in figure. Multi-passage tumours included. e , Representative H&E and IHC for indicated markers in RPM and RPMA sg Ctrl and sgPten tumours with SCLC histopathology versus regions of NSCLC histology including adenocarcinoma (Adeno), adeno-squamous carcinoma (Adeno-squamous), or Squamous differentiation. H-score quantification for indicated proteins (right) in RPM and RPMA “Ctrl” (parental and sgControl -infected tumours, orange) and “ sgPten ” tumours (purple) with SCLC-dominant histopathology (> 50% of tumour region analyzed). Quantification of NSCLC-dominant tumours (only found in RPMA) included on the far right (both “Control” and “ sgPten ” tumours). Exact number of tumours quantified from n = 2–6 mice per genotype indicated in figure. Multi-passage tumours included. f , Stacked bar chart has average proportions of indicated histopathologies in individual RPM and RPMA control or sgPten -tumours. Exact number of tumours analyzed indicated above bars. Error bars, mean ± SEM. Histopathologies determined via analysis of H&E and NKX2-1, P63, KRT5, and SCLC subtype marker staining. LCNEC is large-cell neuroendocrine carcinoma. For gel source data for (a,b), see Supplementary Fig. . All scale bars, 50 μm. All statistical tests are one-way ANOVA tests with Fisher’s LSD multiple comparisons; **** p < 0.0001, ns=not significant= p > 0.05, and other exact p- values indicated in figure.

Techniques: Transformation Assay, Infection, Western Blot, Control, Derivative Assay, Histopathology, Marker, Staining

a-d , Representative tdTom+ cells in naphthalene-injured airway epithelium from Ai9 reporter mice at 3–7 days post-K5-Cre administration stained for indicated basal (KRT5, P63) or hillock (KRT13) markers (upper insets). Bottom panel is merged high magnification co-IF from white box inset indicated in top panel. a , Arrows indicate co-expressing cells in the tracheal epithelium. b , Arrows indicate co-expressing cells in the lung epithelium. c , Arrow indicates tdTom+ cells lacking KRT13 (left) or co-expressing KRT13 in a KRT13 + “hillock” structure (right) in the tracheal epithelium. d , Arrows indicate co-expressing cells in the lung epithelium. e , Percent of tdTom+ cells in the trachea vs lung airway vs total (all airway epithelium) co-expressing or within two cells distance from KRT5 (green), P63 (purple), or KRT13 (turquoise) cells. Quantification reflects all detected tdTom+ cells in mouse airways 3–7 days post K5-Cre administration (n = 4 mice, 160 tdTom+ cells were analyzed for KRT13/tdTom co-stain; n = 90 tdTom+ cells were analyzed for KRT5/P63/tdTom co-stain). Scale bars, 50 µm.

Journal: Nature

Article Title: Basal cell of origin resolves neuroendocrine–tuft lineage plasticity in cancer

doi: 10.1038/s41586-025-09503-z

Figure Lengend Snippet: a-d , Representative tdTom+ cells in naphthalene-injured airway epithelium from Ai9 reporter mice at 3–7 days post-K5-Cre administration stained for indicated basal (KRT5, P63) or hillock (KRT13) markers (upper insets). Bottom panel is merged high magnification co-IF from white box inset indicated in top panel. a , Arrows indicate co-expressing cells in the tracheal epithelium. b , Arrows indicate co-expressing cells in the lung epithelium. c , Arrow indicates tdTom+ cells lacking KRT13 (left) or co-expressing KRT13 in a KRT13 + “hillock” structure (right) in the tracheal epithelium. d , Arrows indicate co-expressing cells in the lung epithelium. e , Percent of tdTom+ cells in the trachea vs lung airway vs total (all airway epithelium) co-expressing or within two cells distance from KRT5 (green), P63 (purple), or KRT13 (turquoise) cells. Quantification reflects all detected tdTom+ cells in mouse airways 3–7 days post K5-Cre administration (n = 4 mice, 160 tdTom+ cells were analyzed for KRT13/tdTom co-stain; n = 90 tdTom+ cells were analyzed for KRT5/P63/tdTom co-stain). Scale bars, 50 µm.

Techniques: Staining, Expressing

Review

goat anti human p63 tp73l polyclonal antibody (R&D Systems)

Structured Review

Images

1) Product Images from "Molecular mechanism of resistance to lonafarnib conferred by mutations in the cysteine-rich region of respiratory syncytial virus fusion glycoprotein and discovery of a lonafarnib-derived antiviral PROTAC"

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